| 摘要： | Skin aging in diabetic patients is closely associated with delayed wound healing and oxidative stress-mediated
fibroblast dysfunction. This study investigated the protective and regenerative effects of a water extract of
Phalaenopsis orchid flower (WEPF), an ornamental plant endemic to Taiwan, on high glucose (HG)-induced
cellular senescence in human dermal fibroblasts (CCD-966SK), with a focus on the Nrf2/HO-1 antioxidant
pathway. Cytotoxicity, cellular senescence, and ROS production were respectively assessed using MTT assay,
senescence-associated β-galactosidase (SA-β-gal) staining, and DCFDA-cellular reactive oxygen species
assay. Western blotting and ELISA were used to analyze the cellular senescence-related proteins. Fibroblasts
treated with WEPF under HG conditions exhibited reduced senescence-associated β-galactosidase (SA-β-gal)
activity, lower ROS levels, and attenuated cell cycle arrest. Protein expression profiling revealed suppression
of the p53/p21Waf1, and p16INK4a/Rb pathways and decreased matrix metalloproteinase-1 (MMP-1) expression.
Mechanistically, WEPF exerted its effects by activating the Nrf2/HO-1 axis and restoring the expression of
senescence marker protein-30 (SMP30), thereby promoting fibroblast repair and reducing pro-inflammatory
signaling. These findings support the potential of WEPF as a botanical therapeutic agent for diabetic wound
healing and age-related skin deterioration. |
